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dc.contributor.authorOpperhuizen, Anne-Loes
dc.contributor.authorFoppen, Ewout
dc.contributor.authorJonker, Martijs
dc.contributor.authorWackers, Paul
dc.contributor.authorvan Faassen, Martijn
dc.contributor.authorvan Weeghel, Michel
dc.contributor.authorvan Kerkhof, Linda
dc.contributor.authorFliers, Eric
dc.contributor.authorKalsbeek, Andries
dc.date.accessioned2019-10-21T17:36:32Z
dc.date.available2019-10-21T17:36:32Z
dc.date.issued2019-01-01
dc.identifier.issn1662-4548
dc.identifier.pmid31281239
dc.identifier.doi10.3389/fnins.2019.00647
dc.identifier.urihttp://hdl.handle.net/10029/623352
dc.description.abstractExposure to light at night (LAN) has been associated with serious pathologies, including obesity, diabetes and cancer. Recently we showed that 2 h of LAN impaired glucose tolerance in rats. Several studies have suggested that the autonomic nervous system (ANS) plays an important role in communicating these acute effects of LAN to the periphery. Here, we investigated the acute effects of LAN on the liver transcriptome of male Wistar rats. Expression levels of individual genes were not markedly affected by LAN, nevertheless pathway analysis revealed clustered changes in a number of endocrine pathways. Subsequently, we used selective hepatic denervations [sympathetic (Sx), parasympathetic (Px), total (Tx, i.e., Sx plus Px), sham] to investigate the involvement of the ANS in the effects observed. Surgical removal of the sympathetic or parasympathetic hepatic branches of the ANS resulted in many, but small changes in the liver transcriptome, including a pathway involved with circadian clock regulation, but it clearly separated the four denervation groups. On the other hand, analysis of the liver metabolome was not able to separate the denervation groups, and only 6 out of 78 metabolites were significantly up- or downregulated after denervations. Finally, removal of the sympathetic and parasympathetic hepatic nerves combined with LAN exposure clearly modulated the effects of LAN on the liver transcriptome, but left most endocrine pathways unaffected. Conclusion: One-hour light-at-night acutely affects the liver transcriptome. Part of this effect is mediated via the nervous innervation, as a hepatectomy modulated and reduced the effect of LAN on liver transcripts.en_US
dc.language.isoenen_US
dc.subjectbiological clocken_US
dc.subjectcircadianen_US
dc.subjectmetabolomeen_US
dc.subjectneural pathwayen_US
dc.subjecttranscriptomeen_US
dc.titleEffects of Light-at-Night on the Rat Liver - A Role for the Autonomic Nervous System.en_US
dc.typeArticleen_US
dc.identifier.journalFront Neurosc 2019; 13:647en_US
dc.source.journaltitleFrontiers in neuroscience


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