Show simple item record

dc.contributor.authorBosch, Thijs
dc.contributor.authorSchade, Rogier
dc.contributor.authorLandman, Fabian
dc.contributor.authorSchouls, Leo
dc.contributor.authorDijk, Karin van
dc.date.accessioned2019-10-21T21:04:49Z
dc.date.available2019-10-21T21:04:49Z
dc.date.issued2019-09-01
dc.identifier.issn1746-0921
dc.identifier.pmid31625443
dc.identifier.doi10.2217/fmb-2019-0091
dc.identifier.urihttp://hdl.handle.net/10029/623411
dc.description.abstractAim: To show that a strain of Aeromonas hydrophila became resistant to carbapenems by interspecies transfer of a plasmid using long-read sequencing. Material & methods: Whole genome sequencing of the four isolates was done using Illumina Hiseq, while the plasmid was reconstructed using the MinION sequencer. The resistome was identified with ResFinder. Results: Whole genome sequencing and long-read sequencing showed that all isolates carried a blaVIM-1 gene located on a 165 kb incA/C plasmid. ResFinder confirmed that the resistome of the plasmid, comprising 13 resistance genes, was identical within all isolates. Discussion: Long-read sequencing using the MinION successfully reconstructed a plasmid that was identical in all isolates, providing evidence for horizontal gene transfer of this blaVIM-1 gene carrying plasmid within the patient.en_US
dc.language.isoenen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectaeromonasen_US
dc.subjectcarbapenemaseen_US
dc.subjectlong-read sequencingen_US
dc.subjectminionen_US
dc.subjectplasmid transferen_US
dc.titleA VIM-1 positive strain in a near-drowning patient: evidence for interspecies plasmid transfer within the patient.en_US
dc.typeArticleen_US
dc.identifier.journalFuture Microbiol 2019; 14:1191-7en_US
dc.source.journaltitleFuture microbiology


This item appears in the following Collection(s)

Show simple item record