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dc.contributor.authorLiu, Dingyu
dc.contributor.authorWagner, James G
dc.contributor.authorMariman, Rob
dc.contributor.authorHarkema, Jack R
dc.contributor.authorGerlofs-Nijland, Miriam E
dc.contributor.authorPinelli, Elena
dc.contributor.authorFolkerts, Gert
dc.contributor.authorCassee, Flemming R
dc.contributor.authorVandebriel, Rob J
dc.date.accessioned2020-07-17T09:29:54Z
dc.date.available2020-07-17T09:29:54Z
dc.date.issued2020-05-01
dc.identifier.issn1091-7691
dc.identifier.pmid32571132
dc.identifier.doi10.1080/08958378.2020.1781986
dc.identifier.urihttp://hdl.handle.net/10029/623991
dc.description.abstractBackground: Inhalation exposure to biological particulate matter (BioPM) from livestock farms may provoke exacerbations in subjects suffering from allergy and asthma. The aim of this study was to use a murine model of allergic asthma to determine the effect of BioPM derived from goat farm on airway allergic responses.Methods: Fine (<2.5 μm) BioPM was collected from an indoor goat stable. Female BALB/c mice were ovalbumin (OVA) sensitized and challenged with OVA or saline as control. The OVA and saline groups were divided in sub-groups and exposed intranasally to different concentrations (0, 0.9, 3, or 9 μg) of goat farm BioPM. Bronchoalveolar lavage fluid (BALF), blood and lung tissues were collected.Results: In saline-challenged mice, goat farm BioPM induced 1) a dose-dependent increase in neutrophils in BALF and 2) production of macrophage inflammatory protein-3a. In OVA-challenged mice, BioPM induced 1) inflammatory cells in BALF, 2) OVA-specific Immunoglobulin (Ig)G1, 3) airway mucus secretion-specific gene expression. RNAseq analysis of lungs indicates that neutrophil chemotaxis and oxidation-reduction processes were the representative genomic pathways in saline and OVA-challenged mice, respectively.Conclusions: A single exposure to goat farm BioPM enhanced airway inflammation in both saline and OVA-challenged allergic mice, with neutrophilic response as Th17 disorder and eosinophilic response as Th2 disorder indicative of the severity of allergic responses. Identification of the mode of action by which farm PM interacts with airway allergic pathways will be useful to design potential therapeutic approaches.en_US
dc.language.isoenen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBioPMen_US
dc.subjectTh17en_US
dc.subjectTh2en_US
dc.subjectallergic airway diseaseen_US
dc.subjectgenomic pathwaysen_US
dc.subjectgoat farmen_US
dc.subjectmurineen_US
dc.subjectovalbuminen_US
dc.titleAirborne particulate matter from goat farm increases acute allergic airway responses in mice.en_US
dc.typeArticleen_US
dc.identifier.journalInhal Toxicol 2020; 32(6):265-77en_US
dc.source.journaltitleInhalation toxicology


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