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dc.contributor.authorLambert, Eleonora E
dc.contributor.authorCorbière, Véronique
dc.contributor.authorvan Gaans-van den Brink, Jacqueline A M
dc.contributor.authorDuijst, Maxime
dc.contributor.authorVenkatasubramanian, Prashanna Balaji
dc.contributor.authorSimonetti, Elles
dc.contributor.authorHuynen, Martijn
dc.contributor.authorDiavatopoulos, Dimitri D
dc.contributor.authorVersteegen, Pauline
dc.contributor.authorBerbers, Guy A M
dc.contributor.authorMascart, Françoise
dc.contributor.authorvan Els, Cécile A C M
dc.date.accessioned2020-08-14T11:39:07Z
dc.date.available2020-08-14T11:39:07Z
dc.date.issued2020-05-15
dc.identifier.issn2076-393X
dc.identifier.pmid32429152
dc.identifier.doi10.3390/vaccines8020225
dc.identifier.urihttp://hdl.handle.net/10029/624050
dc.description.abstractTo advance research and development of improved pertussis vaccines, new immunoassays are needed to qualify the outcome of Bordetella pertussis (Bp) specific CD4+ T-cell differentiation. Here, we applied a recently developed whole blood assay to evaluate Bp specific CD4+ T-cell responses. The assay is based on intracellular cytokine detection after overnight in vitro Bp antigen stimulation of diluted whole blood. We show for the first time that CD4+ T-cell memory of Th1, Th2, and Th17 lineages can be identified simultaneously in whole blood. Participants ranging from 7 to 70 years of age with different priming backgrounds of whole-cell pertussis (wP) and acellular pertussis (aP) vaccination were analyzed around an acellular booster vaccination. The assay allowed detection of low frequent antigen-specific CD4+ T-cells and revealed significantly elevated numbers of activated and cytokine-producing CD4+ T-cells, with a significant tendency to segregate recall responses based on primary vaccination background. A stronger Th2 response hallmarked an aP primed cohort compared to a wP primed cohort. In conclusion, analysis of Bp specific CD4+ T-cell responses in whole blood showed separation based on vaccination background and provides a promising tool to assess the quantity and quality of CD4+ T-cell responses induced by vaccine candidates.en_US
dc.language.isoenen_US
dc.subjectBordetella pertussisen_US
dc.subjectCD4+ T-cellsen_US
dc.subjectTh cytokinesen_US
dc.subjectTh polarizationen_US
dc.subjectbooster vaccinationen_US
dc.subjectvaccination backgrounden_US
dc.subjectwhole blood assayen_US
dc.titleUncovering Distinct Primary Vaccination-Dependent Profiles in Human Specific CD4+ T-Cell Responses Using a Novel Whole Blood Assay.en_US
dc.typeArticleen_US
dc.identifier.journalVaccines 2020; 8(2):pii.e225en_US
dc.source.journaltitleVaccines


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