Uncovering Distinct Primary Vaccination-Dependent Profiles in Human Specific CD4+ T-Cell Responses Using a Novel Whole Blood Assay.
dc.contributor.author | Lambert, Eleonora E | |
dc.contributor.author | Corbière, Véronique | |
dc.contributor.author | van Gaans-van den Brink, Jacqueline A M | |
dc.contributor.author | Duijst, Maxime | |
dc.contributor.author | Venkatasubramanian, Prashanna Balaji | |
dc.contributor.author | Simonetti, Elles | |
dc.contributor.author | Huynen, Martijn | |
dc.contributor.author | Diavatopoulos, Dimitri D | |
dc.contributor.author | Versteegen, Pauline | |
dc.contributor.author | Berbers, Guy A M | |
dc.contributor.author | Mascart, Françoise | |
dc.contributor.author | van Els, Cécile A C M | |
dc.date.accessioned | 2020-08-14T11:39:07Z | |
dc.date.available | 2020-08-14T11:39:07Z | |
dc.date.issued | 2020-05-15 | |
dc.identifier.issn | 2076-393X | |
dc.identifier.pmid | 32429152 | |
dc.identifier.doi | 10.3390/vaccines8020225 | |
dc.identifier.uri | http://hdl.handle.net/10029/624050 | |
dc.description.abstract | To advance research and development of improved pertussis vaccines, new immunoassays are needed to qualify the outcome of Bordetella pertussis (Bp) specific CD4+ T-cell differentiation. Here, we applied a recently developed whole blood assay to evaluate Bp specific CD4+ T-cell responses. The assay is based on intracellular cytokine detection after overnight in vitro Bp antigen stimulation of diluted whole blood. We show for the first time that CD4+ T-cell memory of Th1, Th2, and Th17 lineages can be identified simultaneously in whole blood. Participants ranging from 7 to 70 years of age with different priming backgrounds of whole-cell pertussis (wP) and acellular pertussis (aP) vaccination were analyzed around an acellular booster vaccination. The assay allowed detection of low frequent antigen-specific CD4+ T-cells and revealed significantly elevated numbers of activated and cytokine-producing CD4+ T-cells, with a significant tendency to segregate recall responses based on primary vaccination background. A stronger Th2 response hallmarked an aP primed cohort compared to a wP primed cohort. In conclusion, analysis of Bp specific CD4+ T-cell responses in whole blood showed separation based on vaccination background and provides a promising tool to assess the quantity and quality of CD4+ T-cell responses induced by vaccine candidates. | en_US |
dc.language.iso | en | en_US |
dc.subject | Bordetella pertussis | en_US |
dc.subject | CD4+ T-cells | en_US |
dc.subject | Th cytokines | en_US |
dc.subject | Th polarization | en_US |
dc.subject | booster vaccination | en_US |
dc.subject | vaccination background | en_US |
dc.subject | whole blood assay | en_US |
dc.title | Uncovering Distinct Primary Vaccination-Dependent Profiles in Human Specific CD4+ T-Cell Responses Using a Novel Whole Blood Assay. | en_US |
dc.type | Article | en_US |
dc.identifier.journal | Vaccines 2020; 8(2):pii.e225 | en_US |
dc.source.journaltitle | Vaccines |