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dc.contributor.authorHendrickx, Antoni P A
dc.contributor.authorLandman, Fabian
dc.contributor.authorde Haan, Angela
dc.contributor.authorBorst, Dyogo
dc.contributor.authorWitteveen, Sandra
dc.contributor.authorvan Santen-Verheuvel, Marga G
dc.contributor.authorvan der Heide, Han G J
dc.contributor.authorSchouls, Leo M
dc.date.accessioned2020-10-13T07:30:45Z
dc.date.available2020-10-13T07:30:45Z
dc.date.issued2020-10-08
dc.identifier.issn2045-2322
dc.identifier.pmid33033293
dc.identifier.doi10.1038/s41598-020-73440-2
dc.identifier.urihttp://hdl.handle.net/10029/624439
dc.description.abstractCarbapenemase-producing Klebsiella pneumoniae emerged as a nosocomial pathogen causing morbidity and mortality in patients. For infection prevention it is important to track the spread of K. pneumoniae and its plasmids between patients. Therefore, the major aim was to recapitulate the contents and diversity of the plasmids of genetically related K. pneumoniae strains harboring the beta-lactamase gene blaKPC-2 or blaKPC-3 to determine their dissemination in the Netherlands and the former Dutch Caribbean islands from 2014 to 2019. Next-generation sequencing was combined with long-read third-generation sequencing to reconstruct 22 plasmids. wgMLST revealed five genetic clusters comprised of K. pneumoniae blaKPC-2 isolates and four clusters consisted of blaKPC-3 isolates. KpnCluster-019 blaKPC-2 isolates were found both in the Netherlands and the Caribbean islands, while blaKPC-3 cluster isolates only in the Netherlands. Each K. pneumoniae blaKPC-2 or blaKPC-3 cluster was characterized by a distinct resistome and plasmidome. However, the large and medium plasmids contained a variety of antibiotic resistance genes, conjugation machinery, cation transport systems, transposons, toxin/antitoxins, insertion sequences and prophage-related elements. The small plasmids carried genes implicated in virulence. Thus, implementing long-read plasmid sequencing analysis for K. pneumoniae surveillance provided important insights in the transmission of a KpnCluster-019 blaKPC-2 strain between the Netherlands and the Caribbean.en_US
dc.language.isoenen_US
dc.titlePlasmid diversity among genetically related Klebsiella pneumoniae bla and bla isolates collected in the Dutch national surveillance.en_US
dc.typeArticleen_US
dc.identifier.journalSci Rep 2020; 10(1):16678en_US
dc.source.journaltitleScientific reports


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