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dc.contributor.authorFischer, Carlo
dc.contributor.authorMögling, Ramona
dc.contributor.authorMelidou, Angeliki
dc.contributor.authorKühne, Arne
dc.contributor.authorOliveira-Filho, Edmilson F
dc.contributor.authorWolff, Thorsten
dc.contributor.authorReiche, Janine
dc.contributor.authorBroberg, Eeva
dc.contributor.authorMeijer, Adam
dc.contributor.authorLeitmeyer, Katrin
dc.contributor.authorDrexler, Jan Felix
dc.contributor.authorReusken, Chantal B E M
dc.date.accessioned2020-12-30T07:54:35Z
dc.date.available2020-12-30T07:54:35Z
dc.date.issued2020-12-09
dc.identifier.pmid33298612
dc.identifier.doi10.1128/JCM.02676-20
dc.identifier.urihttp://hdl.handle.net/10029/624597
dc.description.abstractObjective: During the ongoing coronavirus disease 2019 (COVID-19) outbreak, robust detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a key element for clinical management and to interrupt transmission chains. We organized an external quality assessment (EQA) of molecular detection of SARS-CoV-2 for European expert laboratories.Study design: An EQA panel composed of 12 samples, containing either SARS-CoV-2 at different concentrations to evaluate sensitivity or other respiratory viruses to evaluate specificity of SARS-CoV-2 testing, was distributed to 68 laboratories in 35 countries. Specificity samples included seasonal human coronaviruses hCoV-229E, hCoV-NL63 and hCoV-OC43, as well as MERS-CoV, SARS-CoV and human influenza virus A and B.Conclusion: Sensitivity was variable among laboratories, particularly for low concentration SARS-CoV-2 samples. Results indicated that performance was mostly independent of the selection of specific extraction or PCR methods.en_US
dc.language.isoenen_US
dc.rightsCopyright © 2020 Fischer et al.
dc.titleVariable sensitivity in molecular detection of SARS-CoV-2 in European Expert Laboratories: External Quality Assessment, June - July 2020.en_US
dc.typeArticleen_US
dc.identifier.eissn1098-660X
dc.identifier.journalJ Clin Microbiol 2021; 59(3):e02676-20en_US
dc.source.journaltitleJournal of clinical microbiology
dc.source.countryUnited States


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