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dc.contributor.authorBodewes, Rogier
dc.contributor.authorReijnen, Linda
dc.contributor.authorZwagemaker, Florian
dc.contributor.authorKohl, Robert H G
dc.contributor.authorKerkhof, Jeroen
dc.contributor.authorVeldhuijzen, Irene K
dc.contributor.authorvan Binnendijk, Rob
dc.date.accessioned2021-03-08T18:25:04Z
dc.date.available2021-03-08T18:25:04Z
dc.date.issued2021-03-01
dc.identifier.pmid33662587
dc.identifier.doi10.1016/j.meegid.2021.104794
dc.identifier.urihttp://hdl.handle.net/10029/624755
dc.description.abstractMeasles viruses continue to spread globally, despite the availability of a safe and effective vaccine. Molecular surveillance of measles virus has become an essential tool to demonstrate whether cascades of infections in a certain region or country are the result of endemic spread or the repeatedly introduction of the virus in contained outbreaks. Currently, molecular surveillance of measles viruses worldwide is mainly based on 450 nucleotides of the C-terminal region of the nucleoprotein (N450). However, as a result of the disappearance of particular measles virus clades over the past decades, this gene segment does not provide sufficient resolution anymore to answer these questions. To increase the molecular resolution, sequence data were collected from three regions of the measles virus genome, the partial non-coding region between the M and F gene (M-F NCR4465-4754), partial H gene (H8022-8621) and the partial L gene (L10724-11438) for measles viruses detected in 2018 and 2019 in the Netherlands. Analysis of obtained sequence data indicated that sequencing of these three regions resulted in an increase in molecular resolution for measles virus genotype B3 and D8 viruses, two of the four global genotypes currently predominant in the European region. Furthermore, this improved resolution was sufficient to support an epidemiology characterized by repeat introduction of measles virus rather than endemic virus spread. In conclusion, sequencing of the M-F NCR4465-4754, H8022-8621 and L10724-11438 regions of the measles virus is an efficient and useful approach for molecular surveillance of measles viruses.en_US
dc.language.isoenen_US
dc.rightsCopyright © 2021. Published by Elsevier B.V.
dc.subjectMeaslesen_US
dc.subjectMeasles virusen_US
dc.subjectMolecular epidemiologyen_US
dc.subjectMolecular surveillanceen_US
dc.titleAn efficient molecular approach to distinguish chains of measles virus transmission in the elimination phase.en_US
dc.typeArticleen_US
dc.identifier.eissn1567-7257
dc.identifier.journalInfect Genet Evol 2021; 104794en_US
dc.source.journaltitleInfection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
dc.source.beginpage104794
dc.source.endpage
dc.source.countryNetherlands


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