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dc.contributor.authorValcek, Adam
dc.contributor.authorSismova, Petra
dc.contributor.authorNesporova, Kristina
dc.contributor.authorOverballe-Petersen, Søren
dc.contributor.authorBitar, Ibrahim
dc.contributor.authorJamborova, Ivana
dc.contributor.authorKant, Arie
dc.contributor.authorHrabak, Jaroslav
dc.contributor.authorWagenaar, Jaap A
dc.contributor.authorMadec, Jean-Yves
dc.contributor.authorDamborg, Peter
dc.contributor.authorvan Duijkeren, Engeline
dc.contributor.authorEwers, Christa
dc.contributor.authorHordijk, Joost
dc.contributor.authorHasman, Henrik
dc.contributor.authorBrouwer, Michael S M
dc.contributor.authorDolejska, Monika
dc.date.accessioned2021-05-23T17:39:04Z
dc.date.available2021-05-23T17:39:04Z
dc.date.issued2021-02-22
dc.identifier.pmid33619063
dc.identifier.doi10.1128/AAC.02556-20
dc.identifier.urihttp://hdl.handle.net/10029/624974
dc.description.abstractThe relatedness of the equine-associated Escherichia coli ST1250 and its single- and double-locus variants (ST1250-SLV/DLV), obtained from horses in Europe, was studied by comparative genome analysis. A total of 54 isolates of E. coli ST1250 and ST1250-SLV/DLV from healthy and hospitalized horses across Europe [Czech Republic (n=23), the Netherlands (n=18), Germany (n=9), Denmark (n=3) and France (n=1)] from 2008-2017 were subjected to whole-genome sequencing. An additional 25 draft genome assemblies of E. coli ST1250 and ST1250-SLV/DLV were obtained from the public databases. The isolates were compared for genomic features, virulence genes, clade structure and plasmid content. The complete nucleotide sequences of eight IncHI1/ST9 and one IncHI1/ST2 plasmids were obtained using long-read sequencing by PacBio or MinION. In the collection of 79 isolates, only 10 were phylogenetically close (<8 SNP). The majority of isolates belonged to phylogroup B1 (73/79, 92.4%) and carried blaCTX-M-1 (58/79, 73.4%). The plasmid content of the isolates was dominated by IncHI1 of ST9 (56/62, 90.3%) and ST2 (6/62, 9.7%), while 84.5% (49/58) blaCTX-M-1 genes were associated with presence of IncHI1 replicon of ST9 and 6.9% (4/58) with IncHI1 replicon of ST2 within the corresponding isolates. The operon for the utilization of short chain fructooligosaccharides (fos operon) was present in 55 (55/79, 69.6%) isolates, and all of these carried IncHI1/ST9 plasmids. The eight complete IncHI1/ST9 plasmid sequences showed the presence of blaCTX-M-1 and the fos operon within the same molecule. Sequences of IncHI1/ST9 plasmids were highly conserved (>98% similarity) regardless of country of origin and varied only in the structure and integration site of MDR region. E. coli ST1250 and ST1250-SLV/DLV are phylogenetically-diverse strains associated with horses. A strong linkage of E. coli ST1250 with epidemic multi-drug resistance plasmid lineage IncHI1/ST9 carrying blaCTX-M-1 and the fos operon was identified.en_US
dc.language.isoenen_US
dc.rightsCopyright © 2021 American Society for Microbiology.
dc.titleHorsing around: ST1250 of equine origin harbouring epidemic IncHI1/ST9 plasmid with and an operon for short-chain fructooligosaccharides metabolism.en_US
dc.typeArticleen_US
dc.identifier.eissn1098-6596
dc.identifier.journalAntimicrob Agents Chemother 2021; 65(5):e02556-20en_US
dc.source.journaltitleAntimicrobial agents and chemotherapy
dc.source.countryUnited States


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