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dc.contributor.authorArends, D W
dc.contributor.authorMiellet, W R
dc.contributor.authorLangereis, J D
dc.contributor.authorEderveen, T H A
dc.contributor.authorvan der Gaast-de Jongh, C E
dc.contributor.authorvan Scherpenzeel, M
dc.contributor.authorKnol, M J
dc.contributor.authorvan Sorge, N M
dc.contributor.authorLefeber, D J
dc.contributor.authorTrzciński, K
dc.contributor.authorSanders, E A M
dc.contributor.authorDorfmueller, H C
dc.contributor.authorBootsma, H J
dc.contributor.authorde Jonge, M I
dc.date.accessioned2021-07-19T11:51:46Z
dc.date.available2021-07-19T11:51:46Z
dc.date.issued2021-07-12
dc.identifier.pmid34251291
dc.identifier.doi10.1128/IAI.00246-21
dc.identifier.urihttp://hdl.handle.net/10029/625152
dc.description.abstractStreptococcus pneumoniae serotype 19A prevalence has increased after implementation of PCV7 and PCV10 vaccines. In this study, we have provided, with high accuracy, the genetic diversity of the 19A serotype in a cohort of Dutch invasive pneumococcal disease patients and asymptomatic carriers obtained in the period 2004-2016. Whole genomes of the 338 pneumococcal isolates in this cohort were sequenced and their capsule (cps) loci compared to examine the diversity and determine the impact on the production of CPS sugar precursors and CPS shedding. We discovered 79 types with a unique CPS locus sequence. Most variation was observed in the rmlB and rmlD genes of the TDP-Rha synthesis pathway, and in the wzg gene, of unknown function. Interestingly, gene variation in the cps locus was conserved in multiple alleles. Using RmlB and RmlD protein models, we predict that enzymatic function is not affected by the single nucleotide polymorphisms as identified. To determine if RmlB and RmlD function was affected, we analyzed nucleotide sugar levels using UHPLC-MS. CPS precursors differed between 19A cps locus subtypes, including TDP-Rha, but no clear correlation was observed. Also, a significant difference in multiple nucleotide sugar levels was observed between phylogenetically branched groups. Because of indications of a role for Wzg in capsule shedding, we analyzed if this was affected. No clear indication of a direct role in shedding was found. We thus describe genotypic variety in rmlB, rmlD and wzg in serotype 19A the Netherlands, for which we have not discovered an associated phenotype.en_US
dc.language.isoenen_US
dc.titleExamining the distribution and impact of single nucleotide polymorphisms in the capsular locus of serotype 19A.en_US
dc.typeArticleen_US
dc.identifier.eissn1098-5522
dc.identifier.journalInfect Immun 2021; 89(11):e0024621en_US
dc.source.journaltitleInfection and immunity
dc.source.beginpageIAI0024621
dc.source.endpage
dc.source.countryUnited States


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