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dc.contributor.authorRuskovska, Tatjana
dc.contributor.authorBeekhof, Piet
dc.contributor.authorVelickova, Nevenka
dc.contributor.authorKamcev, Nikola
dc.contributor.authorJansen, Eugène
dc.date.accessioned2021-07-19T11:53:04Z
dc.date.available2021-07-19T11:53:04Z
dc.date.issued2021-07-12
dc.identifier.pmid34251957
dc.identifier.doi10.1080/10715762.2021.1942464
dc.identifier.urihttp://hdl.handle.net/10029/625153
dc.description.abstractVarious biomarkers of oxidative stress and redox status have been used in a number of clinical and epidemiological studies related to diseases and conditions that involve disturbances of the redox balance. However, a comprehensive study of diurnal variations of a set of biomarkers has not been conducted so far. Therefore, the aim of this study is to investigate circadian rhythm and time-of-day-effects of a set of frequently used biomarkers of oxidative stress, redox and antioxidant status in serum/plasma. These biomarkers include Reactive Oxygen Metabolites (ROM), Biological Antioxidant Potency (BAP), Total Thiols in Proteins (TTP), high-sensitive C-Reactive Protein (CRP) and Uric Acid (UA). During a 24-hr study, blood sampling was conducted 6 times at 4-hr intervals. The presence of circadian rhythm was analyzed with CircWave analysis, whereas the effect of time was analyzed with Repeated Measures ANOVA (RM-ANOVA). Thereby, the main focus was on the time points in working hours (8, 12 and 16 hr), which are used frequently in practice. Of all investigated biomarkers, only TTP in males demonstrated statistically significant circadian rhythm (p = 0.040). A statistically significant effect between all six time points with RM-ANOVA was observed for ROM, TTP and UA in both genders, and for BAP in females only. No statistically significant differences were observed between the time points 8 hr and 12 hr for any of the biomarkers that were assessed in our study. In conclusion, diurnal variations in some of the studied biomarkers that we demonstrate here should be taken into account when designing and conducting clinical and epidemiological studies. It is advised to standardize the time of sampling with a preference in the morning hours.en_US
dc.language.isoenen_US
dc.subjectSerum biomarkersen_US
dc.subjectdiurnal variationen_US
dc.subjectoxidative stressen_US
dc.subjectplasma biomarkersen_US
dc.subjectredox statusen_US
dc.titleCircadian rhythm and time-of-day-effects of (anti)oxidant biomarkers for epidemiological studies.en_US
dc.typeArticleen_US
dc.identifier.eissn1029-2470
dc.identifier.journalFree Radic Res 2021; 55(7):792-8en_US
dc.source.journaltitleFree radical research
dc.source.beginpage1
dc.source.endpage7
dc.source.countryEngland


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