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dc.contributor.authorWise, Stephen A
dc.contributor.authorCamara, Johanna E
dc.contributor.authorBurdette, Carolyn Q
dc.contributor.authorHahm, Grace
dc.contributor.authorNalin, Federica
dc.contributor.authorKuszak, Adam J
dc.contributor.authorMerkel, Joyce
dc.contributor.authorDurazo-Arvizu, Ramón A
dc.contributor.authorWilliams, Emma L
dc.contributor.authorPopp, Christian
dc.contributor.authorBeckert, Christian
dc.contributor.authorSchultess, Jan
dc.contributor.authorVan Slooten, Glen
dc.contributor.authorTourneur, Carole
dc.contributor.authorPease, Camille
dc.contributor.authorKaul, Ravi
dc.contributor.authorVillarreal, Alfredo
dc.contributor.authorBatista, Marcelo Cidade
dc.contributor.authorPham, Heather
dc.contributor.authorBennett, Alex
dc.contributor.authorJansen, Eugene
dc.contributor.authorKhan, Dilshad Ahmed
dc.contributor.authorKilbane, Mark
dc.contributor.authorTwomey, Patrick J
dc.contributor.authorFreeman, James
dc.contributor.authorParker, Neil
dc.contributor.authorMushtaq, Sohail
dc.contributor.authorSimpson, Christine
dc.contributor.authorLukas, Pierre
dc.contributor.authorCavalier, Étienne
dc.contributor.authorSempos, Christopher T
dc.date.accessioned2021-09-13T12:47:34Z
dc.date.available2021-09-13T12:47:34Z
dc.date.issued2021-08-25
dc.identifier.pmid34435207
dc.identifier.doi10.1007/s00216-021-03577-0
dc.identifier.urihttp://hdl.handle.net/10029/625257
dc.description.abstractAn interlaboratory comparison study was conducted by the Vitamin D Standardization Program (VDSP) to assess the performance of ligand binding assays (Part 2) for the determination of serum total 25-hydroxyvitamin D [25(OH)D]. Fifty single-donor samples were assigned target values for concentrations of 25-hydroxyvitamin D2 [25(OH)D2], 25-hydroxyvitamin D3 [25(OH)D3], 3-epi-25-hydroxyvitamin D3 [3-epi-25(OH)D3], and 24R,25-dihydroxyvitamin D3 [24R,25(OH)2D3] using isotope dilution liquid chromatography-tandem mass spectrometry (ID LC-MS/MS). VDSP Intercomparison Study 2 Part 2 includes results from 17 laboratories using 32 ligand binding assays. Assay performance was evaluated using mean % bias compared to the assigned target values and using linear regression analysis of the test assay mean results and the target values. Only 50% of the ligand binding assays achieved the VDSP criterion of mean % bias ≤ |± 5%|. For the 13 unique ligand binding assays evaluated in this study, only 4 assays were consistently within ± 5% mean bias and 4 assays were consistently outside ± 5% mean bias regardless of the laboratory performing the assay. Based on multivariable regression analysis using the concentrations of individual vitamin D metabolites in the 50 single-donor samples, most assays underestimate 25(OH)D2 and several assays (Abbott, bioMérieux, DiaSorin, IDS-EIA, and IDS-iSYS) may have cross-reactivity from 24R,25(OH)2D3. The results of this interlaboratory study represent the most comprehensive comparison of 25(OH)D ligand binding assays published to date and is the only study to assess the impact of 24R,25(OH)2D3 content using results from a reference measurement procedure.en_US
dc.language.isoenen_US
dc.rights© 2021. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.
dc.subject24R,25-Dihydroxyvitamin D3 [24R,25(OH)2D3]en_US
dc.subject25-Hydroxyvitamin D2 [25(OH)D2]en_US
dc.subject25-Hydroxyvitamin D3 [25(OH)D3]en_US
dc.subjectLigand binding assayen_US
dc.subjectLiquid chromatography–tandem mass spectrometry (LC-MS/MS)en_US
dc.subjectTotal 25-hydroxyvitamin D [25(OH)D]en_US
dc.titleInterlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 - Part 2 ligand binding assays - impact of 25-hydroxyvitamin D and 24R,25-dihydroxyvitamin D on assay performance.en_US
dc.typeArticleen_US
dc.identifier.eissn1618-2650
dc.identifier.journalAnal Bioanal Chem 2022; 414(1):351-366en_US
dc.source.journaltitleAnalytical and bioanalytical chemistry
dc.source.countryGermany


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