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    Establishment of a genotyping scheme for Coxiella burnetii.

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    Authors
    Svraka, Sanela
    Toman, Rudolf
    Skultety, Ludovit
    Slaba, Katarina
    Homan, Wieger L
    Type
    Article
    Language
    en
    
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    Title
    Establishment of a genotyping scheme for Coxiella burnetii.
    Publiekssamenvatting
    Coxiella burnetii is the causative agent of Q fever. The bacterium is highly infectious and is classified as a category B biological weapon. The tools of molecular biology are of utmost importance in a rapid and unambiguous identification of C. burnetii in naturally occurring Q fever outbreaks, or in cases of a deliberate release of the infectious agent. In this work, development of a multiple locus variable number tandem repeats (VNTR) analysis (MLVA) for the characterization of C. burnetii is described. Sixteen C. burnetii isolates and five passage history/laboratory variants were characterized. The VNTR markers revealed many polymorphisms resulting in nine unique MLVA types that cluster into five different clusters. This proves that the MLVA system is highly discriminatory. The selected VNTR markers were stable. The MLVA method developed in this report is a promising tool for the characterization of C. burnetii isolates and their epidemiological study.
    DOI
    10.1111/j.1574-6968.2005.00036.x
    PMID
    16445755
    URI
    http://hdl.handle.net/10029/6919
    ae974a485f413a2113503eed53cd6c53
    10.1111/j.1574-6968.2005.00036.x
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