High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.
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Authors
Aires-de-Sousa, MartaBoye, K
Lencastre, H de
Deplano, A
Enright, M C
Etienne, J
Friedrich, A
Harmsen, D
Holmes, A
Huijsdens, Xander W
Kearns, A M
Mellmann, A
Meugnier, H
Rasheed, J K
Spalburg, Emile
Strommenger, B
Struelens, M J
Tenover, F C
Thomas, J
Vogel, U
Westh, H
Xu, J
Witte, W
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ArticleLanguage
en
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High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.Publiekssamenvatting
Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.PMID
16455927ae974a485f413a2113503eed53cd6c53
10.1128/JCM.44.2.619-621.2006
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