High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authors
Aires-de-Sousa, MartaBoye, K
Lencastre, H de
Deplano, A
Enright, M C
Etienne, J
Friedrich, A
Harmsen, D
Holmes, A
Huijsdens, Xander W
Kearns, A M
Mellmann, A
Meugnier, H
Rasheed, J K
Spalburg, Emile
Strommenger, B
Struelens, M J
Tenover, F C
Thomas, J
Vogel, U
Westh, H
Xu, J
Witte, W
Type
ArticleLanguage
en
Metadata
Show full item recordTitle
High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.Publiekssamenvatting
Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.PMID
16455927ae974a485f413a2113503eed53cd6c53
10.1128/JCM.44.2.619-621.2006
Scopus Count
Collections