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    High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.

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    Authors
    Aires-de-Sousa, Marta
    Boye, K
    Lencastre, H de
    Deplano, A
    Enright, M C
    Etienne, J
    Friedrich, A
    Harmsen, D
    Holmes, A
    Huijsdens, Xander W
    Kearns, A M
    Mellmann, A
    Meugnier, H
    Rasheed, J K
    Spalburg, Emile
    Strommenger, B
    Struelens, M J
    Tenover, F C
    Thomas, J
    Vogel, U
    Westh, H
    Xu, J
    Witte, W
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    Type
    Article
    Language
    en
    
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    Title
    High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.
    Publiekssamenvatting
    Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.
    DOI
    10.1128/JCM.44.2.619-621.2006
    PMID
    16455927
    URI
    http://hdl.handle.net/10029/7094
    ae974a485f413a2113503eed53cd6c53
    10.1128/JCM.44.2.619-621.2006
    Scopus Count
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