Measurement of antibodies to avian influenza virus A(H7N7) in humans by hemagglutination inhibition test.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Kamp, Esther E H M van de
Du Ry van Beest Holle, Mirna
Koopmans, Marion P G
MetadataShow full item record
TitleMeasurement of antibodies to avian influenza virus A(H7N7) in humans by hemagglutination inhibition test.
PubliekssamenvattingDuring the epizootic of highly pathogenic avian influenza A(H7N7) in 2003 in The Netherlands, RT-PCR and culture confirmed infection was detected in 89 persons who were ill. A modified hemagglutination inhibition (HI) test using horse erythrocytes and 2 hemagglutinating units of virus was applied to assess retrospectively the extent of human (subclinical) infection. Validation of the HI-test with sera from 34 RT-PCR and culture confirmed A(H7) infected persons and sera from 100 persons from a human influenza vaccine trial in autumn 2002 showed that this HI-test had a sensitivity of 85% and a specificity of 100% when using a cut-off titer of > or =10. Using this cut-off value, A(H7) specific antibodies were detected in 49% of 508 persons exposed to poultry and in 64% of 63 persons exposed to A(H7) infected persons. Correlation of seropositivity with the occurrence of eye symptoms in exposed persons who had not received antiviral prophylaxis and of reduced seropositivity with taking antiviral prophylaxis provided further evidence that the A(H7) HI antibody titers were real. In conclusion, by applying an HI-test using horse erythrocytes human antibodies against the avian A(H7N7) virus were detected with high sensitivity and specificity in an unexpectedly high proportion of exposed persons.
- Increased sensitivity for detecting avian influenza-specific antibodies by a modified hemagglutination inhibition assay using horse erythrocytes.
- Authors: Jia N, Wang SX, Liu YX, Zhang PH, Zuo SQ, Lin-Zhan, Dang RL, Ma YH, Zhang C, Zhang L, Lu S, Cao WC
- Issue date: 2008 Oct
- Antigenic Fingerprinting of Antibody Response in Humans following Exposure to Highly Pathogenic H7N7 Avian Influenza Virus: Evidence for Anti-PA-X Antibodies.
- Authors: Khurana S, Chung KY, Coyle EM, Meijer A, Golding H
- Issue date: 2016 Oct 15
- Serological reports of human infections of H7 and H9 avian influenza viruses in northern China.
- Authors: Jia N, de Vlas SJ, Liu YX, Zhang JS, Zhan L, Dang RL, Ma YH, Wang XJ, Liu T, Yang GP, Wen QL, Richardus JH, Lu S, Cao WC
- Issue date: 2009 Mar
- Protective measures and human antibody response during an avian influenza H7N3 outbreak in poultry in British Columbia, Canada.
- Authors: Skowronski DM, Li Y, Tweed SA, Tam TW, Petric M, David ST, Marra F, Bastien N, Lee SW, Krajden M, Brunham RC
- Issue date: 2007 Jan 2
- [Hemagglutination inhibition test for retrospective diagnosis of avian influenza in mammals].
- Authors: Kurskaia OG, Durymanov AG, Sharshov KA, Zaĭkovskaia AV, Shestopalov AM, Il'icheva TN
- Issue date: 2009 Sep-Oct