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    Tenth CRL-Salmonella interlaboratory comparison study (2005) on typing of Salmonella spp.

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    Authors
    Korver H
    Maas HME
    Ward LR
    Mevius DJ
    Mooijman KA
    Series/Report no.
    RIVM rapport 330300009
    RIVM rapport 330300009
    Type
    Report
    Language
    en
    
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    Title
    Tenth CRL-Salmonella interlaboratory comparison study (2005) on typing of Salmonella spp.
    Translated Title
    Tiende CRL-Salmonella ringonderzoek (2005) voor de typering van Salmonella spp.
    Publiekssamenvatting
    The tenth interlaboratory comparison study on the typing of Salmonella was organised by the Community Reference Laboratory for Salmonella (CRL-Salmonella, Bilthoven, The Netherlands) in collaboration with the Health Protection Agency (HPA, London, United Kingdom) and the Central Institute for Animal Disease Control (CIDC, Lelystad, The Netherlands) in March 2005. Twenty-six National Reference Laboratories for Salmonella (NRLs-Salmonella), including Norway and 14 Enter-Net Laboratories (ENLs), participated in the study. In total, 20 strains of the species Salmonella enterica subspecies enterica were selected for serotyping. Ten strains of Salmonella Enteritidis (SE) and 10 strains of Salmonella Typhimurium (STM) were selected for phage typing. Ten strains of Salmonella spp. were selected for antimicrobial susceptibility testing. In general, no problems were encountered with the typing of the O antigens. Ninety-nine per cent of the NRLs and 100 % of the ENLs were able to correctly type the O antigens. A few laboratories had problems typing the H antigens. The H antigens were typed correctly by 97% of the NRLs and by 99% of the ENLs. Ninety-four per cent of the NRLs and 99% of the ENLs indicated correct serovar names for the 20 serotyping strains. The phage typing results of the majority of the laboratories were found to be good. This was also valid for the quality of the antimicrobial susceptibility testing of both the NRLs and the ENLs.
    Het tiende ringonderzoek voor de typering van Salmonella werd in maart 2005 georganiseerd door het Communautair Referentie Laboratorium voor Salmonella (CRL-Salmonella, Bilthoven, Nederland) in samenwerking met de Health Protection Agency (HPA, Londen, Verenigd Koninkrijk) en het Centraal Instituut voor Dierziekte Controle (CIDC, Lelystad, Nederland). Zesentwintig Nationale Referentie Laboratoria voor Salmonella (NRLs-Salmonella) inclusief Noorwegen en 14 Enter-Net Laboratoria (ENLs) namen deel aan de studie. Twintig stammen van species Salmonella enterica subspecies enterica werden geselecteerd voor de serotypering. Tien stammen van Salmonella Enteritidis (SE) en 10 stammen van Salmonella Typhimurium (STM) werden geselecteerd voor faagtypering. Tien stammen van Salmonella spp. werden geselecteerd voor antimicrobiele gevoeligheidsbepalingen. In het algemeen werden geen problemen gevonden met de typering van de O-antigenen. Negenennegentig procent van de NRLs en 100 % van de ENLs typeerden de O-antigenen correct. Slechts enkele laboratoria hadden problemen met het typeren van de H-antigenen. De H-antigenen werden correct getypeerd door 97 % van de NRLs en door 99 % van de ENLs. Vierennegentig procent van de NRLs en 99 % van de ENLs gaven de 20 serotyperingsstammen de goede serovar naam. De meeste laboratoria vonden goede resultaten met de faagtypering. Ook de kwaliteit van de antimicrobiele gevoeligheidsbepalingen uitgevoerd door zowel de NRLs als door de ENLs was goed.
    Publisher
    Rijksinstituut voor Volksgezondheid en Milieu RIVM
    URI
    http://hdl.handle.net/10029/7322
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