Genotyping of Giardia in Dutch patients and animals: a phylogenetic analysis of human and animal isolates.
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TitleGenotyping of Giardia in Dutch patients and animals: a phylogenetic analysis of human and animal isolates.
PubliekssamenvattingGiardia duodenalis (syn. Giardia lamblia, Giardia intestinalis) is a protozoan organism that can infect the intestinal tract of many animal species including mammals. Genetic heterogeneity of G. duodenalis is well described but the zoonotic potential is still not clear. In this study, we analysed 100 Giardia DNA samples directly isolated from human stool specimens, to get more insight in the different G. duodenalis assemblages present in the Dutch human population. Results showed that these human isolates could be divided into two main Assemblages A and B within the G. duodenalis group on the basis of PCR assays specific for the Assemblages A and B and the DNA sequences of 18S ribosomal RNA and the glutamate dehydrogenase (gdh) genes. Genotyping results showed that G. duodenalis isolates originating from Dutch human patients belonged in 35% of the cases to Assemblage A (34/98) and in 65% of the cases to Assemblage B (64/98) whereas two human cases remained negative in all assays tested. In addition, we compared these human samples with animal samples from the Netherlands and human and animal samples from other countries. A phylogenetic analysis was carried out on the DNA sequences obtained from these Giardia and those available in GenBank. Using gdh DNA sequence analysis, human and animal Assemblage A and B Giardia isolates could be identified. However, phylogenetic analysis revealed different sub-clustering for human and animal isolates where host-species-specific assemblages (C, D, E, F and G) could be identified. The geographic origin of the human and animal samples was not a discriminating factor.
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- Authors: Monis PT, Andrews RH, Mayrhofer G, Mackrill J, Kulda J, Isaac-Renton JL, Ey PL
- Issue date: 1998 Jan
- [Occurrence of Giardia species and genotypes in humans and animals in Wielkopolska region, Poland].
- Authors: Solarczyk P
- Issue date: 2009
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- Authors: Volotão AC, Costa-Macedo LM, Haddad FS, Brandão A, Peralta JM, Fernandes O
- Issue date: 2007 Apr
- High prevalence Giardia duodenalis assemblage B and potentially zoonotic subtypes in sporadic human cases in Western Australia.
- Authors: Yang R, Lee J, Ng J, Ryan U
- Issue date: 2010 Mar 1
- Risk of human infection with Giardia duodenalis from cats in Japan and genotyping of the isolates to assess the route of infection in cats.
- Authors: Suzuki J, Murata R, Kobayashi S, Sadamasu K, Kai A, Takeuchi T
- Issue date: 2011 Apr