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Validation of ISO 6579-4 for identification of monophasic Salmonella Typhimurium (1,4,[5],12:i:-) by polymerase chain reaction (PCR)
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Series / Report no.
RIVM rapport 2023-0430
Open Access
Type
Report
Language
en
Date
2025-02-10
Research Projects
Organizational Units
Journal Issue
Title
Validation of ISO 6579-4 for identification of monophasic Salmonella Typhimurium (1,4,[5],12:i:-) by polymerase chain reaction (PCR)
Translated Title
Validatie van ISO 6579-4 om monofasische Salmonella Typhimurium te identificeren met PCR
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Abstract
Mensen kunnen ziek worden als de Salmonella-bacterie in voedsel zit. De overheid heeft daarom wetgeving gemaakt om voedsel hierop te controleren. Er bestaan meer dan 2600 verschillende typen Salmonella. Van enkele typen die relatief vaak voorkomen worden mensen het vaakst ziek. De wetgeving is daarom strenger voor deze typen Salmonella. Eén daarvan is monofasische Salmonella Typhimurium. Er zijn typen die veel op monofasische Salmonella Typhimurium lijken. De huidige methode om ze op te sporen, kan ze niet van elkaar onderscheiden. Daarvoor is een specifieke methode nodig: de PCR (polymerase chain reaction). De Internationale Standaardisatie Organisatie (ISO) heeft een standaard ontwikkeld om monofasische Salmonella Typhimurium met drie PCRmethoden te herkennen. Voor deze nieuwe standaard moet worden gecontroleerd of deze drie PCR-methoden goed werken. Het RIVM heeft dat gecontroleerd met een zogeheten validatiestudie. Daaruit bleek dat alle drie de PCR-methoden goed in staat zijn om monofasische Salmonella Typhimurium te typeren. De referentiemethode is beschikbaar onder nummer ISO 6579-4. Het Europese Unie Referentie Laboratorium voor Salmonella (EURLSalmonella) heeft deze validatiestudie gedaan. Het Nationale Referentie Laboratoria voor Salmonella (NRL-Salmonella) uit Duitsland heeft hier aan meegewerkt. Het EURL-Salmonella is gevestigd bij het RIVM.
People can become ill if the bacterium Salmonella is present in food. Therefore, the government has made legislation to monitor food for this. There are more than 2 600 different types of Salmonella. A few types are found more frequently and cause most of the illnesses. Legislation is more stringent for these Salmonella types. One of these is monophasic Salmonella Typhimurium. Some types look a lot like monophasic Salmonella Typhimurium. The current method for detection cannot distinguish between these types. A specific method is needed for that purpose: the polymerase chain reaction (PCR). The International Organization for Standardization (ISO) has developed a standard for identification of monophasic Salmonella Typhimurium with three PCR methods. The three PCR methods for this new standard have to be checked for their performance. The Dutch National Institute for Public Health and the Environment (RIVM) has checked this by means of a 'validation study'. This showed that all three PCR methods are well able to type monophasic Salmonella Typhimurium. This reference method is available under number ISO 6579-4. The European Union Reference Laboratory for Salmonella (EURLSalmonella) conducted this validation study. The German National Reference Laboratory for Salmonella (NRL-Salmonella) also participated in this study. The EURL-Salmonella is situated at RIVM.
People can become ill if the bacterium Salmonella is present in food. Therefore, the government has made legislation to monitor food for this. There are more than 2 600 different types of Salmonella. A few types are found more frequently and cause most of the illnesses. Legislation is more stringent for these Salmonella types. One of these is monophasic Salmonella Typhimurium. Some types look a lot like monophasic Salmonella Typhimurium. The current method for detection cannot distinguish between these types. A specific method is needed for that purpose: the polymerase chain reaction (PCR). The International Organization for Standardization (ISO) has developed a standard for identification of monophasic Salmonella Typhimurium with three PCR methods. The three PCR methods for this new standard have to be checked for their performance. The Dutch National Institute for Public Health and the Environment (RIVM) has checked this by means of a 'validation study'. This showed that all three PCR methods are well able to type monophasic Salmonella Typhimurium. This reference method is available under number ISO 6579-4. The European Union Reference Laboratory for Salmonella (EURLSalmonella) conducted this validation study. The German National Reference Laboratory for Salmonella (NRL-Salmonella) also participated in this study. The EURL-Salmonella is situated at RIVM.
Description
Publisher
Rijksinstituut voor Volksgezondheid en Milieu RIVM