Limited influence of hospital wastewater on the microbiome and resistome of wastewater in a community sewerage system.

2.50
Hdl Handle:
http://hdl.handle.net/10029/621953
Title:
Limited influence of hospital wastewater on the microbiome and resistome of wastewater in a community sewerage system.
Authors:
Buelow, Elena; Bayjanov, Jumamurat R; Majoor, Eline; Willems, Rob J L; Bonten, Marc J M; Schmitt, Heike; van Schaik, Willem
Abstract:
Effluents from wastewater treatment plants (WWTPs) have been proposed to act as point sources of antibiotic-resistant bacteria (ARB) and antimicrobial resistance genes (ARGs) in the environment. Hospital sewage may contribute to the spread of ARB and ARGs as it contains the feces and urine of hospitalized patients, who are more frequently colonized with multi-drug resistant bacteria than the general population. However, whether hospital sewage noticeably contributes to the quantity and diversity of ARGs in the general sewerage system has not yet been determined.Here, we employed culture-independent techniques, namely 16S rRNA gene sequencing and nanolitre-scale quantitative PCRs, to assess the role of hospital effluent as a point source of ARGs in the sewerage system, through comparing microbiota composition and levels of ARGs in hospital sewage with WWTP influent with and without hospital sewage.Compared to other sites, hospital sewage was richest in human-associated bacteria and contained the highest relative levels of ARGs. Yet, the relative abundance of ARGs was comparable in the influent of WWTPs with and without hospital sewage, suggesting that hospitals do not contribute importantly to the quantity and diversity of ARGs in the investigated sewerage system.
Citation:
Limited influence of hospital wastewater on the microbiome and resistome of wastewater in a community sewerage system. 2018 FEMS Microbiol. Ecol.
Journal:
FEMS Microbiol Ecol 2018; advance online publication (ahead of print)
Issue Date:
14-May-2018
URI:
http://hdl.handle.net/10029/621953
DOI:
10.1093/femsec/fiy087
PubMed ID:
29767712
Type:
Article
Language:
en
ISSN:
1574-6941
Appears in Collections:
Miscellaneous

Full metadata record

DC FieldValue Language
dc.contributor.authorBuelow, Elenaen
dc.contributor.authorBayjanov, Jumamurat Ren
dc.contributor.authorMajoor, Elineen
dc.contributor.authorWillems, Rob J Len
dc.contributor.authorBonten, Marc J Men
dc.contributor.authorSchmitt, Heikeen
dc.contributor.authorvan Schaik, Willemen
dc.date.accessioned2018-05-27T13:08:38Z-
dc.date.available2018-05-27T13:08:38Z-
dc.date.issued2018-05-14-
dc.identifier.citationLimited influence of hospital wastewater on the microbiome and resistome of wastewater in a community sewerage system. 2018 FEMS Microbiol. Ecol.en
dc.identifier.issn1574-6941-
dc.identifier.pmid29767712-
dc.identifier.doi10.1093/femsec/fiy087-
dc.identifier.urihttp://hdl.handle.net/10029/621953-
dc.description.abstractEffluents from wastewater treatment plants (WWTPs) have been proposed to act as point sources of antibiotic-resistant bacteria (ARB) and antimicrobial resistance genes (ARGs) in the environment. Hospital sewage may contribute to the spread of ARB and ARGs as it contains the feces and urine of hospitalized patients, who are more frequently colonized with multi-drug resistant bacteria than the general population. However, whether hospital sewage noticeably contributes to the quantity and diversity of ARGs in the general sewerage system has not yet been determined.Here, we employed culture-independent techniques, namely 16S rRNA gene sequencing and nanolitre-scale quantitative PCRs, to assess the role of hospital effluent as a point source of ARGs in the sewerage system, through comparing microbiota composition and levels of ARGs in hospital sewage with WWTP influent with and without hospital sewage.Compared to other sites, hospital sewage was richest in human-associated bacteria and contained the highest relative levels of ARGs. Yet, the relative abundance of ARGs was comparable in the influent of WWTPs with and without hospital sewage, suggesting that hospitals do not contribute importantly to the quantity and diversity of ARGs in the investigated sewerage system.en
dc.language.isoenen
dc.rightsArchived with thanks to FEMS microbiology ecologyen
dc.titleLimited influence of hospital wastewater on the microbiome and resistome of wastewater in a community sewerage system.en
dc.typeArticleen
dc.identifier.journalFEMS Microbiol Ecol 2018; advance online publication (ahead of print)en

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