van Gorkom, Tamaravan Arkel, Gijs H JVoet, WillemThijsen, Steven F TKremer, Kristin2021-05-232021-05-232021-05-123398065110.1128/JCM.03280-20http://hdl.handle.net/10029/624984The diagnosis of Lyme neuroborreliosis (LNB) is based on neurological symptoms, cerebrospinal fluid (CSF) pleocytosis, and intrathecally produced Borrelia-specific antibodies. In most cases, the presence of intrathecally produced Borrelia-specific antibodies is determined by using an enzyme-linked immunosorbent assay (ELISA). The edge-effect is a known phenomenon in ELISAs and can negatively influence the assay reproducibility, repeatability, as well as index calculations of sample pairs which are tested in the same run.enCopyright © 2021 van Gorkom et al.Article1098-660XJ Clin Microbiol 2021; 59(8):e0328020