Bosch, ThijsSchade, RogierLandman, FabianSchouls, LeoDijk, Karin van2019-10-212019-10-212019-09-011746-09213162544310.2217/fmb-2019-0091http://hdl.handle.net/10029/623411Aim: To show that a strain of Aeromonas hydrophila became resistant to carbapenems by interspecies transfer of a plasmid using long-read sequencing. Material & methods: Whole genome sequencing of the four isolates was done using Illumina Hiseq, while the plasmid was reconstructed using the MinION sequencer. The resistome was identified with ResFinder. Results: Whole genome sequencing and long-read sequencing showed that all isolates carried a blaVIM-1 gene located on a 165 kb incA/C plasmid. ResFinder confirmed that the resistome of the plasmid, comprising 13 resistance genes, was identical within all isolates. Discussion: Long-read sequencing using the MinION successfully reconstructed a plasmid that was identical in all isolates, providing evidence for horizontal gene transfer of this blaVIM-1 gene carrying plasmid within the patient.eninfo:eu-repo/semantics/closedAccessaeromonascarbapenemaselong-read sequencingminionplasmid transferArticleFuture Microbiol 2019; 14:1191-7