Boxman, Ingeborg L AJansen, Claudia C CHägele, GekeZwartkruis-Nahuis, AnsCremer, JeroenVennema, HarryTijsma, Aloys S L2018-01-032018-01-032017-09-18Porcine blood used as ingredient in meat productions may serve as a vehicle for hepatitis E virus transmission. 2017, 257:225-231 Int. J. Food Microbiol.1879-34602869289010.1016/j.ijfoodmicro.2017.06.029http://hdl.handle.net/10029/620995The aim of the present study was to investigate whether the use of porcine blood(products) in food could be a risk for a hepatitis E virus (HEV) infection. HEV RNA was detected in 33/36 batches of (non-heated) liquid products and in 7/24 spray dried powder products. Contamination levels varied among the products, but were highest in liquid whole blood, plasma and fibrinogen reaching levels of 2.2×102 to 2.8×102 HEV genome copies per 0.2g. Sequence analyses revealed genotype 3 strains, of which two were 100% (493nt) identical to recently diagnosed HEV cases, although no direct epidemiological link was established. The industry provided information on processing of blood products in (ready-to-eat)-meat. From this, it was concluded that blood products as an ingredient of processed meat may not be sufficiently heated prior to consumption, and therefore could be a vehicle for transmission.enArchived with thanks to International journal of food microbiologyAnimalsBlood-Borne PathogensFemaleFood ContaminationGenotypeHepatitis EHepatitis E virusHumansMeatMeat ProductsNetherlandsRNA, ViralSwineSwine DiseasesArticleInt J Food Microbiol 2017, 257:225-31