It Takes Two to Tango: Combining Conventional Culture With Molecular Diagnostics Enhances Accuracy of Detection and Pneumococcal Serogroup/Serotype Determination in Carriage.

Miellet, Willem Rvan Veldhuizen, JaniekeLitt, DavidMariman, RobWijmenga-Monsuur, Alienke JBadoux, PaulNieuwenhuijsen, TessaThombre, RebeccaMayet, SanaaEletu, SeyiSheppard, Carmenvan Houten, Marianne AliceRots, Nynke YMiller, ElizabethFry, Norman KSanders, Elisabeth A MTrzciński, Krzysztof2022-05-092022-05-092022-04-181664-302X3550931410.3389/fmicb.2022.859736http://hdl.handle.net/10029/625763The specificity of molecular methods for the detection of Streptococcus pneumoniae carriage is under debate. We propose a procedure for carriage surveillance and vaccine impact studies that increases the accuracy of molecular detection of live pneumococci in polymicrobial respiratory samples.enCopyright © 2022 Miellet, van Veldhuizen, Litt, Mariman, Wijmenga-Monsuur, Badoux, Nieuwenhuijsen, Thombre, Mayet, Eletu, Sheppard, van Houten, Rots, Miller, Fry, Sanders and Trzciński.Streptococcus pneumoniae (pneumococcus)carriageconventional culturediagnostic accuracyqPCR (quantitative PCR)It Takes Two to Tango: Combining Conventional Culture With Molecular Diagnostics Enhances Accuracy of Detection and Pneumococcal Serogroup/Serotype Determination in Carriage.ArticleFrontiers in microbiology 2022;13:859736