Significant Effects of Maternal Diet During Pregnancy on the Murine Fetal Brain Transcriptome and Offspring Behavior.
Edlow, Andrea G Guedj, Faycal Sverdlov, Deanna Pennings, Jeroen L A Bianchi, Diana W
Edlow, Andrea G
Guedj, Faycal
Sverdlov, Deanna
Pennings, Jeroen L A
Bianchi, Diana W
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Series / Report no.
Open Access
Type
Article
Language
en
Date of publication
2019-01-01
Year of publication
Research Projects
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Journal Issue
Title
Significant Effects of Maternal Diet During Pregnancy on the Murine Fetal Brain Transcriptome and Offspring Behavior.
Translated Title
Published in
Front Neurosci 2019; 13:1335
Abstract
Throughout pregnancy and lactation, female C57Bl/6J mice were fed one of two standard, commercially available chow diets (pellet versus powder). The powdered chow diet was relatively deficient in micronutrients and enriched for carbohydrates and n-3 long-chain polyunsaturated fatty acids compared to the pelleted chow. RNA was extracted from embryonic day 15.5 forebrains and hybridized to whole genome expression microarrays (N = 5/maternal diet group). Functional analyses of significantly differentially expressed fetal brain genes were performed using Ingenuity Pathways Analysis and Gene Set Enrichment Analysis. Neonatal behavior was assessed using a validated scale (N = 62 pellet-exposed and 31 powder-exposed). Hippocampal learning, locomotor behavior, and motor coordination were assessed in a subset of adults using fear conditioning, open field testing, and Rotarod tests (N = 16 pellet-exposed, 14 powder-exposed).