Design, development, and testing of a new multi-locus sequence typing scheme for the zoonotic pathogen
Troell, Karin; Stensvold, Christen Rune; Sannella, Anna Rosa; Betson, Martha; Östlund, Emma; Chalmers, Rachel M; Chaudhry, Umer; Davidson, Rebecca; Davies, Lauren; Ignatius, Ralf; de Jong, Anton; Karadjian, Gregory; Adjou, Karim; Klotz, Christian; Ptochos, Sokratis; Robinson, Guy; Roelfsema, Jeroen; Soba, Barbara; Sroka, Jacek; Vatta, Paolo; Wensman, Jonas Johansson; Cacciò, Simone M
Series / Report no.
Open Access
Type
Journal Article
Article
Article
Language
en
Date
2025-08-14
Research Projects
Organizational Units
Journal Issue
Title
Design, development, and testing of a new multi-locus sequence typing scheme for the zoonotic pathogen
Translated Title
Published in
Curr Res Parasitol Vector Borne Dis 2025; 8:100308
Abstract
The zoonotic parasite is an important global cause of diarrheal disease in humans and young ruminants. Molecular typing is essential to track transmission routes and identify clusters of cases. Here, we developed a novel multi-locus sequence typing (MLST) scheme based on single nucleotide polymorphisms (SNPs) in unlinked markers. Coding regions with high variability were identified by comparing whole genome sequences (WGS) from 43 human- and 92 ruminant-derived samples collected across Europe. We first selected 18 markers and showed that they provide high discrimination among the samples with WGS data, with 88% of the MLSTs being singletons. Next, we defined a MLST based on eight genetically unlinked markers and generated sequence data from 305 samples, collected from four different host species and 13 European countries. We consolidated a set of 365 fully genotyped samples, characterized by the presence of 154 different MLSTs, 105 of which were singletons. Network analyses showed no complete clustering of samples by host species or country of origin at the European scale. We further showed that samples with subtypes that are common in Europe are divided into many MLSTs by the new scheme, highlighting its increased discriminatory ability. However, the applicability of the scheme in public health settings is limited by its cost, turnaround time, and scalability. To achieve discrimination of samples based on SNPs, a large number of loci needs to be analysed, and this is feasible using amplicon sequencing technologies.