The consequences of the edge-effect in a commercial enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Lyme neuroborreliosis.
van Gorkom, Tamara van Arkel, Gijs H J Voet, Willem Thijsen, Steven F T Kremer, Kristin
van Gorkom, Tamara
van Arkel, Gijs H J
Voet, Willem
Thijsen, Steven F T
Kremer, Kristin
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Series / Report no.
Open Access
Type
Article
Language
en
Date of publication
2021-05-12
Year of publication
Research Projects
Organizational Units
Journal Issue
Title
The consequences of the edge-effect in a commercial enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Lyme neuroborreliosis.
Translated Title
Published in
J Clin Microbiol 2021; 59(8):e0328020
Abstract
The diagnosis of Lyme neuroborreliosis (LNB) is based on neurological symptoms, cerebrospinal fluid (CSF) pleocytosis, and intrathecally produced Borrelia-specific antibodies. In most cases, the presence of intrathecally produced Borrelia-specific antibodies is determined by using an enzyme-linked immunosorbent assay (ELISA). The edge-effect is a known phenomenon in ELISAs and can negatively influence the assay reproducibility, repeatability, as well as index calculations of sample pairs which are tested in the same run.