A tool to assess the mock community samples in 16S rRNA gene-based microbiota profiling studies.
Shetty, Sudarshan A Kool, Jolanda Fuentes, Susana
Shetty, Sudarshan A
Kool, Jolanda
Fuentes, Susana
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Series / Report no.
Open Access
Type
Article
Language
en
Date of publication
2023-04-27
Year of publication
Research Projects
Organizational Units
Journal Issue
Title
A tool to assess the mock community samples in 16S rRNA gene-based microbiota profiling studies.
Translated Title
Published in
Microbiome Res Rep 2023;2(2):14
Abstract
Inclusion and investigation of technical controls in microbiome sequencing studies is important for understanding technical biases and errors. Here, we present chkMocks, a general R-based tool that allows researchers to compare the composition of mock communities that are processed along with samples to their theoretical composition. A visual comparison between experimental and theoretical community composition and their correlation is provided for researchers to assess the quality of their sample processing workflows.