Quantification of Salmonella Survival and Infection in an In vitro Model of the Human Intestinal Tract as Proxy for Foodborne Pathogens.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsWijnands, Lucas M
Teunis, Peter F M
Kuijpers, Angelina F A
Delfgou-Van Asch, Ellen H M
MetadataShow full item record
TitleQuantification of Salmonella Survival and Infection in an In vitro Model of the Human Intestinal Tract as Proxy for Foodborne Pathogens.
Published inFront Microbiol 2017, 8:1139
PubliekssamenvattingDifferent techniques are available for assessing differences in virulence of bacterial foodborne pathogens. The use of animal models or human volunteers is not expedient for various reasons; the use of epidemiological data is often hampered by lack of crucial data. In this paper, we describe a static, sequential gastrointestinal tract (GIT) model system in which foodborne pathogens are exposed to simulated gastric and intestinal contents of the human digestive tract, including the interaction of pathogens with the intestinal epithelium. The system can be employed with any foodborne bacterial pathogens. Five strains of Salmonella Heidelberg and one strain of Salmonella Typhimurium were used to assess the robustness of the system. Four S. Heidelberg strains originated from an outbreak, the fifth S. Heidelberg strain and the S. Typhimurium strain originated from routine meat inspections. Data from plate counts, collected for determining the numbers of surviving bacteria in each stage, were used to quantify both the experimental uncertainty and biological variability of pathogen survival throughout the system. For this, a hierarchical Bayesian framework using Markov chain Monte Carlo (MCMC) was employed. The model system is able to distinguish serovars/strains for in vitro infectivity when accounting for within strain biological variability and experimental uncertainty.
- Assessing phenotypic virulence of Salmonella enterica across serovars and sources.
- Authors: Petrin S, Wijnands L, Benincà E, Mughini-Gras L, Delfgou-van Asch EHM, Villa L, Orsini M, Losasso C, Olsen JE, Barco L
- Issue date: 2023
- Genomic Analysis of Salmonella enterica Serovar Typhimurium Characterizes Strain Diversity for Recent U.S. Salmonellosis Cases and Identifies Mutations Linked to Loss of Fitness under Nitrosative and Oxidative Stress.
- Authors: Hayden HS, Matamouros S, Hager KR, Brittnacher MJ, Rohmer L, Radey MC, Weiss EJ, Kim KB, Jacobs MA, Sims-Day EH, Yue M, Zaidi MB, Schifferli DM, Manning SD, Walson JL, Miller SI
- Issue date: 2016 Mar 8
- [Applications of simulated gastro-intestinal model in foodborne pathogens: tolerance and pathogenesis].
- Authors: Wang S, Zhang Z, Mu L, Liu H, Pan Y, Zhao Y
- Issue date: 2018 Jun 25
- Horizontal Gene Transfer and Acquired Antibiotic Resistance in Salmonella enterica Serovar Heidelberg following In Vitro Incubation in Broiler Ceca.
- Authors: Oladeinde A, Cook K, Lakin SM, Woyda R, Abdo Z, Looft T, Herrington K, Zock G, Lawrence JP, Thomas JC 4th, Beaudry MS, Glenn T
- Issue date: 2019 Nov 15
- Comparison of methods for quantitating Salmonella enterica Typhimurium and Heidelberg strain attachment to reusable plastic shipping container coupons and preliminary assessment of sanitizer efficacy.
- Authors: Shi Z, Baker CA, Lee SI, Park SH, Kim SA, Ricke SC
- Issue date: 2016 Sep