Bacteriological detection of Salmonella in the presence of competitive micro-organisms. Bacteriological collaborative study IV amongst the National Reference Laboratories for Salmonella, the use of MSRV as selective enrichment
Citations
Altmetric:
Series / Report no.
Open Access
Type
Report
Language
en
Date
2000-04-20
Research Projects
Organizational Units
Journal Issue
Title
Bacteriological detection of Salmonella in the
presence of competitive micro-organisms. Bacteriological collaborative study
IV amongst the National Reference Laboratories for Salmonella, the use of
MSRV as selective enrichment
Translated Title
Bacteriologische detectie van Salmonella in
aanwezigheid van stoorflora. Bacteriologisch ringonderzoek IV voor de
Nationale Referentie Laboratoria voor Salmonella, het gebruik van MSRV als
selectieve ophoping
Published in
Abstract
Het Communautair Referentie Laboratorium voor
Salmonella heeft een vierde bacteriologisch ringonderzoek georganiseerd
betreffende de detectie van Salmonella. De deelnemers waren de Nationale
Referentie Laboratoria (NRLs) voor Salmonella uit de lidstaten van de
Europese Unie. Dit ringonderzoek had twee doelen: 1) Evaluatie van de
resultaten van verschillende besmettingsniveaus van Salmonella Enteritidis
(100 en 1000 kve) en Salmonella Typhimurium (10 en 100 kve) in de
aanwezigheid van competitieve flora tussen en binnen de NRLs; en 2)
Evaluatie van MSRV als selectieve ophoping vergeleken met de standaard
methode welke RV als selectieve ophoping heeft. De gebruikte methoden waren
een aangepaste ISO 6579 (voorgestelde referentie) methode en, optioneel, de
methode die door het laboratorium routinematig gebruikt wordt voor detectie
van Salmonella in kippen feces. Significant meer positieve isolaties werden
verkregen bij de STM100 capsules ten opzichte van de STM10 en SE1000
capsules. Deze laatste twee capsulesoorten behaalden onderling ongeveer
eenzelfde detectieniveau. Het aantal positieve isolaties verkregen met de
SE100 capsules was significant lager dan het aantal positieven verkregen met
de STM10 en SE1000 capsules. Significant meer positieven werden gevonden
met het gebruik van MSRV ten opzichte van RV.
A fourth bacteriological collaborative study was organised by the Community Reference Laboratory for Salmonella. All National Reference Laboratories for Salmonella (NRLs) participated. This study had two objectives: 1) Evaluation of the results of the detection of different contamination levels of Salmonella Enteritidis (100 and 1000 cfp) and Salmonella Typhimurium (10 and 100 cfp) in the presence of competitive micro-organisms among and within the NRLs; and 2)Evaluation of MSRV as selective enrichment compared to the standard method using RV as selective enrichment. Methods used were an adapted ISO 6579 (proposed reference) method and, optionally, the laboratory's own routine method for the detection of Salmonella in chicken faeces. Significantly more positive isolations were obtained with the STM100 capsules compared to the STM10 and SE1000 capsules. These last two capsules showed the same detection level. The number of positive isolations from the SE100 capsules was significantly lower than the number of positive isolations from the STM10 and SE1000 capsules. Significantly more positive isolations were revealed when using MSRV than when using RV.
A fourth bacteriological collaborative study was organised by the Community Reference Laboratory for Salmonella. All National Reference Laboratories for Salmonella (NRLs) participated. This study had two objectives: 1) Evaluation of the results of the detection of different contamination levels of Salmonella Enteritidis (100 and 1000 cfp) and Salmonella Typhimurium (10 and 100 cfp) in the presence of competitive micro-organisms among and within the NRLs; and 2)Evaluation of MSRV as selective enrichment compared to the standard method using RV as selective enrichment. Methods used were an adapted ISO 6579 (proposed reference) method and, optionally, the laboratory's own routine method for the detection of Salmonella in chicken faeces. Significantly more positive isolations were obtained with the STM100 capsules compared to the STM10 and SE1000 capsules. These last two capsules showed the same detection level. The number of positive isolations from the SE100 capsules was significantly lower than the number of positive isolations from the STM10 and SE1000 capsules. Significantly more positive isolations were revealed when using MSRV than when using RV.
Description
Publisher
Sponsors
European Commission
Legislation Veterinaire et Zootechnique
Legislation Veterinaire et Zootechnique