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dc.contributor.authorWubs KL
dc.contributor.authorStolker AAM
dc.contributor.authorGinkel LA van
dc.date.accessioned2012-12-12T15:08:04Z
dc.date.available2012-12-12T15:08:04Z
dc.date.issued1993-06-30
dc.identifier319002002
dc.identifier.urihttp://hdl.handle.net/10029/257136
dc.description.abstractAbstract niet beschikbaar
dc.description.abstractIn this report a study is described on the fully automated determination of desmethyldiazepam in rat serum. Isolation of desmethyldiazepam from rat serum is with robotized solid-phase extraction with a High-Hydrophobic Octadecylsilane sorbent by using the ASPEC (Automatic Sample Preparation with Extraction Columns.) The solid-phase purified extract is, after dilution, injected on a chromatographic system. Subsequent clean-up of the extract is by column-switching. A "heart-cut" of the precolumn eluate containing the relevant part of the extract (desmethyldiazepam and the internal standard prazepam) is directed to the analytical column. Detection is by UV-absorbance detection at 226 nm. The data are collected, stored and processed on an analytical workstation. The extraction recovery (+- standard deviation) is 83,3 +- 1,0)%. The precision is 5,3% at 1,1 mg/l. The method is adapted to a concentration range from 0.05 to 5,0 mg/l starting with 150 mul serum. The resulting method is completed by the introduction of a quality control to assure the quality and the reliability of the results.
dc.description.sponsorshipHIG CBG
dc.format.extent56 p
dc.language.isonl
dc.relation.ispartofRIVM Rapport 319002002
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/319002002.html
dc.subject18nl
dc.subjectnordazepamnl
dc.subjectseranl
dc.subjectanalysenl
dc.subjectautomatiseringnl
dc.subjectextractienl
dc.subjecthplcnl
dc.subjectnordazepamen
dc.subjectseraen
dc.subjectanalysisen
dc.subjectautomationen
dc.subjectextractionen
dc.subjecthplcen
dc.subjectsolid-phase extractionen
dc.subjectuv-detectionen
dc.subjectultraviolet-detectieen
dc.titleVolledig geautomatiseerde bepaling van desmethyldiazepam in ratteserum met behulp van gerobotiseerde vaste-fase extractie en hogeprestatie vloeistofchromatografie met ultraviolet-detectienl
dc.title.alternative[Fully automated determination of desmethyldiazepam in rat serum by robotized solid-phase extraction and high-performance liquid chromatography with UV-detection.]en
dc.typeReport
dc.date.updated2012-12-12T15:08:06Z
html.description.abstractAbstract niet beschikbaar
html.description.abstractIn this report a study is described on the fully automated determination of desmethyldiazepam in rat serum. Isolation of desmethyldiazepam from rat serum is with robotized solid-phase extraction with a High-Hydrophobic Octadecylsilane sorbent by using the ASPEC (Automatic Sample Preparation with Extraction Columns.) The solid-phase purified extract is, after dilution, injected on a chromatographic system. Subsequent clean-up of the extract is by column-switching. A "heart-cut" of the precolumn eluate containing the relevant part of the extract (desmethyldiazepam and the internal standard prazepam) is directed to the analytical column. Detection is by UV-absorbance detection at 226 nm. The data are collected, stored and processed on an analytical workstation. The extraction recovery (+- standard deviation) is 83,3 +- 1,0)%. The precision is 5,3% at 1,1 mg/l. The method is adapted to a concentration range from 0.05 to 5,0 mg/l starting with 150 mul serum. The resulting method is completed by the introduction of a quality control to assure the quality and the reliability of the results.


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