The 20th EURL-Salmonella workshop : 28 and 29 May 2015, Berlin, Germany
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Series/Report no.RIVM report 2015-0083
MetadataShow full item record
TitleThe 20th EURL-Salmonella workshop : 28 and 29 May 2015, Berlin, Germany
Translated TitleDe twintigste EURL-Salmonella workshop : 28 en 29 mei 2015, Berlijn, Duitsland
PubliekssamenvattingHet RIVM heeft de verslagen gebundeld van de presentaties van de twintigste jaarlijkse workshop voor de Europese Nationale Referentie Laboratoria (NRL's) voor de bacterie Salmonella (28 en 29 mei 2015). Het doel van de workshop is dat het overkoepelende orgaan, het Europese Referentie Laboratorium (EURL) Salmonella, en de NRL's informatie kunnen uitwisselen. Daarnaast worden de resultaten gepresenteerd van de ringonderzoeken van het EURL, waarmee de kwaliteit van de NRL-laboratoria wordt aangegeven. Een uitgebreidere weergave van de resultaten wordt per ringonderzoek in aparte RIVMrapporten opgenomen.
Nieuwe technieken steeds belangrijker
Een aantal verslagen geeft informatie over het gebruik van nieuwe technieken om overeenkomsten tussen verschillende Salmonellastammen aan te tonen. Veelal zijn dit moleculaire technieken die het DNA van de bacterie aantonen. Deze technieken worden steeds vaker gebruikt bij het opsporen van de ziekmakende bacterie in voedsel, dieren en bij de mens. Iedere bacteriestam heeft namelijk een eigen unieke moleculaire typering.
Een databank voor unieke moleculaire typering resultaten
De European Food Safety Authority (EFSA) geeft verslag van een databank in oprichting. In deze databank kunnen alle Europese landen moleculaire typeringsresultaten van Salmonella opslaan. Zo is het mogelijk om na te gaan of een bepaalde ziekmakende bacteriestam in meerdere landen en producten voorkomt.
NRL's presenteren hun activiteiten
In vier verslagen wordt informatie gegeven over de activiteiten van de NRL's voor Salmonella uit Noord-Ierland, Portugal, Spanje en Slovakije.
De organisatie van de workshop is in handen van het EURL voor Salmonella, dat onderdeel is van het RIVM. De hoofdtaak van het EURL- Salmonella is toezien op de kwaliteit van de nationale referentielaboratoria voor deze bacterie in Europa.
In this report, the RIVM presents a summary of the presentations given at the 20th annual workshop for the European National Reference Laboratories (NRLs) for Salmonella (28 and 29 May 2015). The aim of this workshop is to facilitate the exchange of information on the activities of the NRLs and the European Union Reference Laboratory for Salmonella (EURL-Salmonella). An important item on the agenda is the presentation of the results of the annual ring trials organised by the EURL, which provide valuable information on the quality of the work carried out by the participating NRL laboratories. Detailed information on the results per ring trial is described in separate RIVM-reports.
New techniques more important
Several presentations provide information on the use of new techniques to show similarities between different Salmonella strains. These are often molecular techniques, analysing the DNA of the bacterium. These techniques are often used to trace the pathogen in food, animals or humans, as each strain has its own unique molecular typing pattern.
A database for unique molecular typing results
The European Food Safety Authority (EFSA) presented their pilot database in which molecular typing results of Salmonella can be stored. This will make it possible to check whether a specific strain is found in different countries and products.
NRLs present their activities
In four presentations information is given of the activities performed by the NRLs for Salmonella of Northern-Ireland, Portugal, Spain and the Slovak Republic.
The annual workshop is organised by the EURL-Salmonella, part of the Dutch National Institute for Public Health and the Environment. The main task of the EURL-Salmonella is to evaluate the performance of the European NRLs in detecting and typing Salmonella in different products.
DescriptionAlleen digitaal verschenen<br>
SponsorsEuropean Commission EC
Showing items related by title, author, creator and subject.
Thirteenth CRL-Salmonella interlaboratory comparison study (2008) on typing of Salmonella spp. : Dertiende CRL-Salmonella ringonderzoek (2008) voor de typering van Salmonella spp.Berk PA; Maas HME; de Pinna E; Mooijman KA; LZO; cib (Rijksinstituut voor Volksgezondheid en Milieu RIVMHealth Protection Agency HPA, 2010-11-04)The National Reference Laboratories (NRLs) of all 27 European Member States performed well on the 2008 quality control test on Salmonella typing. The 4 laboratories which repeated the test also obtained good scores. An analysis of the pooled results from all NRLs revealed that the NRLs taken as a whole were able to assign the correct name to 97 % of the strains tested. One NRL performed the test at a relatively late date and, consequently, its data could not be included in the group analysis. Since 1992, the NRLs have been required to participate in an annual quality control test, which consists of an interlaboratory comparison study for Salmonella typing. Each Member State designates a specific laboratory within their national boundaries to be responsible for the detection and identification of Salmonella strains from samples isolated from animals and/or food products. These laboratories are then referred to as the National Reference Laboratories. The performance of the NRLs is assessed annually based on their capability to correctly identify 20 Salmonella strains. NRLs from countries outside the European Union occasionally participate in these tests, and NRLs from 2 countries belonging to the European Free Trade Association (EFTA) took part in the 2008 test. The expertise of a number of NRLs was subjected to more severe testing by having not only to identify the 20 Salmonella strains of the quality control test but also to subtype (phage typing) various other Salmonella strains. As such, these laboratories received 10 strains of each of Salmonella Enteritidis and Salmonella Typhimurium. These NRLs typed 97 % of the S. Typhimurium strains correctly. The typing of S. Enteritidis strains proved to be more troublesome, with the NRLs typing 94 % of the strains correctly. The Community Reference Laboratory for Salmonella (CRL-Salmonella) organises this annual interlaboratory comparison study in cooperation with the Health Protection Agency in London, UK. The CRL-Salmonella is situated at the National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
Sixteenth EURL-Salmonella interlaboratory comparison study (2011) on typing of Salmonella spp. : Zestiende EURL-Salmonella ringonderzoek (2011) voor de typering van Salmonella spp.Jacobs-Reitsma WF; Pol-Hofstad IE; Maas HME; de Pinna E; Mooijman KA; LZO; cib (Rijksinstituut voor Volksgezondheid en Milieu RIVM, 2012-10-24)The 28 National Reference Laboratories (NRLs) of all 27 European Union (EU) Member States performed well on the 2011 quality control test on Salmonella typing. Two laboratories were found to require a follow-up study on their first test. Altogether, the EU-NRLs were able to assign the correct name to 97% of the strains tested. Other participants interlaboratory comparison study Salmonella Since 1992, the NRLs of the EU Member States have been required to participate in annual quality control tests, which consist of interlaboratory comparison studies on Salmonella. Laboratories from countries outside the European Union, like EU-candidate countries, occasionally participate in these tests on a voluntary basis. Eight additional laboratories participated in the current study. Two EUcandidate countries amongst these eight additional participants did not meet the criteria for good performance in the first round. One of them did not reach this goal in the follow-up study either. The other was not able to participate in the follow-up study; a follow-up study is not compulsory for non-EU laboratories. Each EU Member State designates a specific laboratory within their national boundaries to be responsible for the detection and identification of Salmonella strains from animals and/or food products. These laboratories are then referred to as the National Reference Laboratories. The performance of these NRLs on Salmonella typing is assessed annually, based on their capability to correctly identify 20 Salmonella strains. Phage typing Nine NRLs not only serotyped the 20 Salmonella strains of the quality control test, but also subtyped 20 additional strains by phage typing. For this, the laboratories received ten strains of Salmonella Enteritidis and ten strains of Salmonella Typhimurium. These NRLs typed 98% of the S. Typhimurium strains correctly. Of the S. Enteritidis strains, 88% were phage typed correctly. The European Union Reference Laboratory for Salmonella (EURL-Salmonella) organises this annual interlaboratory comparison study on typing of Salmonella in cooperation with the Health Protection Agency in London, UK. The EURLSalmonella is situated at the National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
Test results of Salmonella typing by the NRLs-Salmonella in the Member States of the EU and the EnterNet Laboratories - Collaborative study VI (2001) on typing of SalmonellaKorver H; Raes M; Maas HME; Ward LR; Wannet WJB; Henken AM; MGB; LIS (PHLS-Colindale/London, 2002-09-06)Test results of Salmonella sero- and phage typing and antimicrobial susceptibility testing by the National Reference Laboratories for Salmonella in the Member States of the European Union and the EnterNet Laboratories: Collaborative study VI (2001) for Salmonella. The sixth collaborative typing study for Salmonella was organised by the Community Reference Laboratory for Salmonella (CRL-Salmonella, Bilthoven, The Netherlands) in collaboration with the Public Health Laboratory Services (PHLS), London, UK. Seventeen National Reference Laboratories for Salmonella (NRLs-Salmonella) and 15 EnterNet laboratories (ENLs) participated in the study. Three of the NRLs for Salmonella are also ENLs. The results of these three NRL-ENL laboratories will only be evaluated with the NRLs for Salmonella. In total, 19 strains of the species Salmonella enterica subsp. enterica and one strain of the species Salmonella enterica subsp. arizonae were selected for serotyping and antimicrobial susceptibility testing, while 10 strains of Salmonella Typhimurium (STM) and 10 strains of Salmonella Enteritidis (SE) were selected for phage typing. In general, no problems were encountered with the typing of the O antigens. However, some laboratories had problems with typing the H antigens. Antimicrobial susceptibility testing revealed data showing that standardisation of this technique would be required to allow for comparison between laboratories. The majority of the EnterNet Laboratories and National Reference Laboratories for Salmonella did not encounter major problems with phage typing of STM and SE strains.